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doi:10.3389/fphar.2016.00433

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Appetite suppression typically intensifies after a dose increase, which is the intended effect

Blagosklonny argued that the metabolic side effects of rapamycin are a consequence of it acting as a CRM and are required to mediate its positive effects on lifespan (226)

For instance, activation of AMPK by metformin has been observed at much lower metformin concentrations in comparable cell culture protocols using metformin concentrations that are within the upper limits of its therapeutic plasma levels (4, 134)