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Progressive glutathione depletion across trimesters : First trimester: 36% LOWER than non-pregnant Second trimester: 55% LOWER Third trimester: 87% LOWER This generates more NAPQI at precisely the time when glutathione reserves are least able to neutralize itcreating maximum vulnerability

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These data suggest that the anticoagulant used for sample collection and time to deproteinization, both independently and additively impact measured levels of free GSH (and thus the ratio of free GSH to GSSG) in samples of plasma which are deproteinized and stored at 80 o C