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Acta Diabetol 51:865873 Gaidhu MP, Fediuc S, Anthony NM et al (2009) Prolonged AICAR-induced AMP-kinase activation promotes energy dissipation in white adipocytes: novel mechanisms integrating HSL and ATGL

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Then the tissue homogenate centrifuged at 2000 rpm/min for 15 min (Centrifuge 5804R, Eppendorf, Germany)